Comparing methods of human precision cut tissue slice culture - tissue slice viability and functional markers data

McGreevy, Owen ORCID: https://orcid.org/0000-0002-2029-0207, Gilbert, Timothy ORCID: https://orcid.org/0000-0003-4821-1897 and Randle, Laura ORCID: https://orcid.org/0000-0002-7881-2979 (2025) Comparing methods of human precision cut tissue slice culture - tissue slice viability and functional markers data. [Data Collection]

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Description

This study examines the viability and functionality of hPCTS using different tissue culture formats. hPCTS were cultured for 15 days using an organotypic polytetrafluoroethylene Millicell insert, a CELLBLOKS® hydrophilic polyethylene terephthalate flow system plate or without an insert. Viability was assessed through MTS assays, while functionality was determined by measuring urea and albumin secretion across the 15 days in culture.

Keywords:	CELLBLOKS®, ex-vivo, human precision cut tissue slices (hPCTS), Millicell inserts, organotypic culture, polyethylene terephthalate (PET) membrane, polytetrafluoroethylene (PTFE) membrane, preclinical model
Divisions:	Faculty of Health & Life Sciences > Inst. Systems, Molec & Integrative Biology > Pharmacology & Therapeutics
Date Deposited:	04 Mar 2025 14:14
Last Modified:	04 Mar 2025 14:14
DOI:	10.17638/datacat.liverpool.ac.uk/2958
Geography:	Liverpool
URI:	https://datacat.liverpool.ac.uk/id/eprint/2958
Additional details Additional details Creators: McGreevy, Owen Gilbert, Timothy Randle, Laura Research funder: Grant Number Name of the Funder NC/X001679 NC3Rs PHD2022.03 NWCR Date: 25 February 2025 Statement on legal, ethical and access issues : Risk of disclosing patient identifiers was mitigated by removing reference to name, date of birth or NHS number. Resource language: English Publisher: University of Liverpool Contact email address: hlomcgre@liverpool.ac.uk Last Modified: 04 Mar 2025 14:14

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Viability Dat ... Rs paper.xlsx Viability Dat ... Rs paper.xlsx Viability Dat ... Rs paper.xlsx Visible to: Anyone Content type: Data Description: MTS viability data for human precision cut tissue slices (hPCTS) cultured over 15 days using three different culture methods (no insert, Millicell insert, CELLBLOCKS®). Metadata Revision: 2 License: Creative Commons: Attribution-Noncommercial-Share Alike 4.0 File size: 51kB Creative Commons: Attribution-Noncommercial-Share Alike 4.0

Urea and Al ... Rs paper.xlsx Urea and Al ... Rs paper.xlsx Urea and Al ... Rs paper.xlsx Visible to: Anyone Content type: Data Description: Percentage change in urea and albumin secretion into media from day 3 in hPCTS cultured over 15 days. Metadata Revision: 3 License: Creative Commons: Attribution-Noncommercial-Share Alike 4.0 File size: 19kB Creative Commons: Attribution-Noncommercial-Share Alike 4.0

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Readme a pr ... e culture.txt Readme a pr ... e culture.txt Readme a pr ... e culture.txt Visible to: Anyone Content type: README file Description: README file description Metadata Revision: 4 License: Creative Commons: Attribution-Noncommercial-Share Alike 4.0 File size: 15kB Creative Commons: Attribution-Noncommercial-Share Alike 4.0

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