Turk-Kubo, Kendra and Mahaffey, Claire (2025) Raw ddPCR abundance of UCYN-A2 and UCYN-A1 from DY167. [Data Collection]
Description
Abundances of B. bigelowii/UCYN-A1 and B. bigelowii//UCYN-A2/A3/A4 were estimated from 11 surface water filtered seawater samples (< 3m, Sterivex 0.22 µm)using digital droplet PCR (ddPCR) using the assays originally developed for Taqman® quantitative PCR by Church et al., (2005) and Thompson et al., (2014), respectively. All reaction conditions, thermocycling parameters, thresholding and calculations of the limit of detection and limit of quantitation are described in Gradoville et al., (2020). Note that the abundances determined using the UCYN-A2/A3/A4 assay likely represent both UCYN-A2 and UCYN-A4, both of which were recovered via nifH amplicon HTS.
| Keywords: | North Sea, Diazotrophs, UCYNA, nifH gene |
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| Date Deposited: | 05 Sep 2025 13:50 |
| Last Modified: | 05 Sep 2025 13:50 |
| DOI: | 10.17638/datacat.liverpool.ac.uk/3040 |
| Geography: | North Sea |
| URI: | https://datacat.liverpool.ac.uk/id/eprint/3040 |
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