Comparing methods of human precision cut tissue slice culture - tissue slice viability and functional markers data

McGreevy, Owen ORCID: https://orcid.org/0000-0002-2029-0207, Gilbert, Timothy ORCID: https://orcid.org/0000-0003-4821-1897 and Randle, Laura ORCID: https://orcid.org/0000-0002-7881-2979 (2025) Comparing methods of human precision cut tissue slice culture - tissue slice viability and functional markers data. [Data Collection]

This is the latest version of this item.

Data Catalogue DOI: 10.17638/datacat.liverpool.ac.uk/2997

Description

This study examines the viability and functionality of hPCTS using different tissue culture formats. hPCTS were cultured for 15 days using an organotypic polytetrafluoroethylene Millicell insert, a CELLBLOKS® hydrophilic polyethylene terephthalate flow system plate or without an insert. Viability was assessed through MTS assays, while functionality was determined by measuring urea and albumin secretion across the 15 days in culture.

Keywords:

CELLBLOKS®, ex-vivo, human precision cut tissue slices (hPCTS), Millicell inserts, organotypic culture, polyethylene terephthalate (PET) membrane, polytetrafluoroethylene (PTFE) membrane, preclinical model

Divisions:

Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology > Pharmacology and Therapeutics

Depositing User:

Owen McGreevy

Date Deposited:

26 Mar 2025 16:42

Last Modified:

26 Mar 2025 16:42

DOI:

10.17638/datacat.liverpool.ac.uk/2997

Geography:

Liverpool