READ ME File For 'Amino acid-based thermoplastic polyurethanes for pelvic floor tissue scaffolds' Date that the readme file was created: 2026/06/12 ------------------- GENERAL INFORMATION ------------------- ReadMe Author: Charlie Bateman, University of Liverpool [0000-0002-1967-9282] Related projects: Amino acid-based thermoplastic polyurethanes for soft tissue repair ------------------- PROJEJCT INFORMATION ------------------- Funder Name: Engineering and Physical Research Council Project Number or Identifier: EP/T518074/1─2442883 and EP/W018977/1 ------------------- CONTACT INFORMATION ------------------- Author information Name: Charlie Bateman ORCID:0000-0002-1967-9282 Institution: University of Liverpool Email:charlie.bateman@liverpool.ac.uk Principal Investigator information Name: Biqiong Chen ORCID: 0000-0002-6465-2871 Institution: University of Liverpool Email:biqiong.chen@liverpool.ac.uk -------------------------- SHARING/ACCESS INFORMATION -------------------------- Licenses/restrictions placed on the data, or limitations of reuse: CC-BY Recommended citation for the data: Use the journal citation below. This dataset supports the publication: AUTHORS: Charlie Bateman, Catherine Fulton, Fionnuala Lundy, Biqiong Chen TITLE: Amino acid-based thermoplastic polyurethanes for pelvic floor tissue scaffolds PAPER DOI IF KNOWN:https://doi.org/10.1002/mame.70353 -------------------- DATA & FILE OVERVIEW -------------------- This dataset contains: File list: Cover page - Information regarding the title and authors of the article MM&E Figure 1 FTIR - FTIR data for polymers MM&E Figure 2b 13C NMR - C NMR data for polymers MM&E Figure 2c 1H NMR - H NMR data for polymers MM&E Figure 3 GPC - GPC data for polymers MM&E Figure 4 DSC - DSC data for polymers MM&E Figure 5a TGA - TGA data for polymers MM&E Figure 5b DTG - DTG data for polymers MM&E Figure 6 TPU Film Tensile - Tensile test data for polymer films MM&E Figure 7 TPU Film Cyclic - Cyclic test data for polymers MM&E Figure 8a TPU Film Swelling - Swelling ratio for polymers MM&E Figure 8b TPU Film Contact Angle - Water contact angle data for polymers MM&E Figure 9 TPU Film Biodegradation - Biodegradation data for polymers MM&E Figure 12 TPU Scaffold Tensile - Tensile test data for scaffolds MM&E Figure 13a TPU Scaffold Cyclic Aligned - Cyclic test data for aligned scaffold structures MM&E Figure 13b TPU Scaffold Cyclic Unaligned - Cyclic test data for unaligned scaffold structures -------------------------- METHODOLOGICAL INFORMATION -------------------------- Description of methods used for collection/generation of data: FT-IR were measured using a Perkin Elmer Spectrum 100 in the range of 650 - 4000 cm-1 over 16 scans using a resolution of 4 cm-1. NMR was measured using a Bruker AVIII400 NMR Spectrometer with CDCL3 mobile phase. Data was analyzed using TopSpin 3.6.3 software. GPC used an Agilent 1260 Infinity II GPC with Agilent GPC/SEC software. Samples were prepared by dissolving the TPU in THF at a concentration of 3 mg.ml-1 and filtered through a PTFE syringe filter with pore size 0.45 µm. The method used 2 x PLgel 5 µm MIXED-C columns (PS/DVB) and 1 x PLgel 5 µm guard column at 35 °C and a flow rate of 1 ml.min-1. The GPC was calibrated using 12 × EasiVial PS-H (2 mL) standards, with number average molecular weights of 162, 580, 1210, 4880, 10330, 22790, 75050, 194500, 479200, 885000, 3152000 and 6570000 g mol−1. DSC used an aluminum pan with approximately 10 mg mass sample for each TPU using the TA Discovery DSC25 with Trios v5 Software. All samples received three heat-cool cycles between −80 and 120 °C under a nitrogen flow (at a rate of 50 ml.min-1), held for 2 minutes between cycles with a heating or cooling rate of 10 °C.min-1. TGA was run at 10 °C.min-1 up to 600 °C under nitrogen flow (at a rate of 50 ml.min-1) using the Netzsch TG 209F1 Libra. The swelling degree of TPUs was found by measuring the initial dry weight and the weight when submerged in PBS solution (pH = 7.4) at 37 °C ± 2 for specific time periods. Samples (n = 5) were removed from the PBS solution and surface liquid was removed with paper towel and immediately weighed. The surface water contact angle of TPU films (n = 3) was measured using a Biolin Scientific Attension Theta Tensiometer. A droplet of water (20 μl) was placed onto the TPU film. Images were captured and angles measured using ImageJ software. Quasi-static tensile tests were performed on film samples with a Lloyds LS5 with a 500 N load cell at a 100 mm.min-1 strain rate until failure with a 2 N preload. Dumbbell samples (n = 5, thickness: 1.8 - 2.1 mm) were cut from films using a die with dimensions specific to ISO 37, Type 3. Quasi-static tensile tests were performed on foam samples with a Lloyds LRX with a 50 N load cell at a 100 mm.min-1 strain rate. Rectangular samples (n = 3, thickness: 2 - 2.2 mm, length 20 mm) were cut from foams using a guillotine to ensure a straight edge was maintained and each end was mounted to a wooden stick during testing to ensure the force is evenly distributed across the sample. Cyclic tensile tests on film samples were performed on a Lloyds LS5 with a 500 N load cell at a 50 mm.min-1 for 5 cycles at a strain of 0 to 10% (n = 5, thickness: 1.8 − 2.1 mm). There was no rest time between two consecutive cycles. Cyclic tensile tests on foam samples were performed on a Lloyds LRX with a 50 N load cell at a 50 mm.min-1 for 5 cycles at a strain of 0 to 10%. Rectangular samples (n = 3, thickness: 2 - 2.2 mm, length 20 mm) were cut from foams using a guillotine and each end was mounted to a wooden stick during testing. In resilience tests 5 cycles are considered acceptable to characterize the overall resilience as this allows for a reliable assessment of how the material can recover after deformation. The density of the solid and foam polymer was measured by weighing the mass of samples using an analytical balance and measuring the dimensions of samples using a digital caliper. Five specimens were tested for each material. In vitro degradation studies were performed on TPU discs (n = 4, thickness: 1.8 - 2.1 mm, diameter: 8 - 10 mm) at 37 °C ± 2 in a shaker incubator (Grant-bio ES-20) at 100 rpm for 180 days. Specimens were saturated in a 70% v/v ethanol-water solution and shaken in the shaker incubator for 15 minutes at 100 rpm. Following this they were washed with distilled water; this procedure was performed three times, and all specimens were dried in a vacuum oven at 37 °C to achieve constant weight (the initial dry weight). To simulate the body conditions for biodegradation studies, PBS solution (pH = 7.4) was prepared by dissolving PBS tablets in distilled water and in the PBS solution containing lipase enzyme at a concentration of 110 U.L-1. The PBS without enzyme was used as a control. The PBS solution with or without enzyme was changed daily for 28 days to ensure the enzyme activity was consistent and after defined incubation days all specimens were removed, washed in distilled water to remove residue and dried in a vacuum oven at 37 °C until a constant weight was achieved. Medium change frequency was reduced to alternating days (day 29 - 90) and twice a week (day 91 - 180) as the study progressed. The weight loss of TPUs was found by measuring the initial dry weight and the new dry weight after submersion in solutions. Methods for processing the data: Software or instrument-specific information needed to interpret the data: Environmental/experimental conditions: each test was carried out at room temperature in a standard environment unless stated otherwise Describe any quality-assurance procedures performed on the data: each of the machines was consistently checked to ensure it was working properly and that it was giving valid data People involved with sample collection, processing, analysis and/or submission: Charlie Bateman