GENERAL INFORMATION:
• Title of Dataset: Raw video files: Caustic signatures produced by gold nanoparticles
• Author information: 
• Name: Genevieve Schleyer
• Institution: University of Liverpool 
• Email: G.Schleyer@Liverpool.ac.uk
• Date of data collection: from 2023-01 to 2023-09
• Geographic location of data collection: Liverpool, UK
• Information about funding sources that supported collection of the data: the author was supported by the BBSRC as part of the collaborative training program.

DATA AND FILE OVERVIEW:
• Methodology:
The files in this data repository are the raw video files used to calculate the diffusion coefficient of gold spherical nanoparticles. Each video contains at least one caustic signature produced by a nanoparticle which was analysed using the ImageJ plugin TrackMate. The microscope used was the Axiocam Observer Zm1 (Carl Zeiss, Oberkochen, Germany). The microscope was set up as described by Patterson and Whelan (2008). The microscope was equipped with an incubation stage (Incubator PM S1, Temp and CO2 module S1, Carl Zeiss, Oberkochen, Germany) which was set at 37C and a 40x objective lens together with a camera (Axiocam z1.m, Carl Zeiss, Oberkochen, Germany) supported by the ZEN software. The selected particle tracks from the video files below were analysed over a period of 100 frames to determine the diffusion coefficient. The acquisition speed of the camera was 40fps. PLEASE NOTE: Over the data collection period, the camera was replaced with a more up to date model. Using the 40x objective lens with the old camera, the pixel size was 0.116΅m x 0.116΅m. This scaling ratio should be assumed for all videos except where indicated with * where the pixel size is 0.086΅m x 0.086΅m.

• Contents of files:
100nm/:
• BPEI-100nm-2.5FBS-Rep-1, BPEI-100nm-2.5FBS-REP-2, BPEI-100nm-2.5FBS-REP-3, BPEI-100nm-2.5FBS-REP-4/: particle tracks recorded for 100nm gold BPEI nanoparticles in DMEM supplemented with 2.5% FBS repeated 4 times (N=4). 6 particles were tracked in each sample (n=6). 
• BPEI-100nm-5FBS-REP-1, BPEI-100nm-5FBS-REP-2, BPEI-100nm-5FBS-REP-3, BPEI-100nm-5FBS-REP-4/: particle tracks recorded for 100nm gold BPEI nanoparticles in DMEM supplemented with 5% FBS repeated 4 times (N=4). 6 particles were tracked in each sample (n=6). 
• BPEI-100nm-10FBS-REP-1, BPEI-100nm-10FBS-REP-2, BPEI-100nm-10FBS-REP-3, BPEI-100nm-10FBS-REP-4/: particle tracks recorded for 100nm gold BPEI nanoparticles in DMEM supplemented with 10% FBS repeated 4 times (N=4). 6 particles were tracked in each sample (n=6). 
• BPEI-100nm-25FBS-REP-1, BPEI-100nm-25FBS-REP-2, BPEI-100nm-25FBS-REP-3, BPEI-100nm-25FBS-REP-4/: particle tracks recorded for 100nm gold BPEI nanoparticles in DMEM supplemented with 25% FBS repeated 4 times (N=4). 6 particles were tracked in each sample (n=6). 
• Citrate-100nm-2.5FBS-REP-1, Citrate -100nm-2.5FBS-REP-2, Citrate -100nm-2.5FBS-REP-3, Citrate -100nm-2.5FBS-REP-4/: particle tracks recorded for 100nm gold Citrate nanoparticles in DMEM supplemented with 2.5% FBS repeated 4 times (N=4). 6 particles were tracked in each sample (n=6). 
• Citrate -100nm-5FBS-REP-1, Citrate -100nm-5FBS-REP-2, Citrate -100nm-5FBS-REP-3, Citrate -100nm-5FBS-REP-4/: particle tracks recorded for 100nm gold Citrate nanoparticles in DMEM supplemented with 5% FBS repeated 4 times (N=4). 6 particles were tracked in each sample (n=6). 
• Citrate -100nm-10FBS-REP-1, Citrate -100nm-10FBS-REP-2, Citrate -100nm-10FBS-REP-3, Citrate -100nm-10FBS-REP-4/: particle tracks recorded for 100nm gold Citrate nanoparticles in DMEM supplemented with 10% FBS repeated 4 times (N=4). 6 particles were tracked in each sample (n=6). 
• Citrate -100nm-25FBS-REP-1, Citrate -100nm-25FBS-REP-2, Citrate -100nm-25FBS-REP-3, Citrate -100nm-25FBS-REP-4/: particle tracks recorded for 100nm gold Citrate nanoparticles in DMEM supplemented with 25% FBS repeated 4 times (N=4). 6 particles were tracked in each sample (n=6). 

50nm/:
• BPEI-50nm-2.5FBS-REP-1, BPEI-50nm-2.5FBS-REP-2, BPEI-50nm-2.5FBS-REP-3*, BPEI-50nm-2.5FBS-REP-4*/: particle tracks recorded for 50nm gold BPEI nanoparticles in DMEM supplemented with 2.5% FBS repeated 4 times (N=4). 6 particles were tracked in each sample (n=6). 
• BPEI-50nm-5FBS-REP-1, BPEI-50nm-5FBS-REP-2, BPEI-50nm-5FBS-REP-3, BPEI-50nm-5FBS-REP-4*/: particle tracks recorded for 50nm gold BPEI nanoparticles in DMEM supplemented with 5% FBS repeated 4 times (N=4). 6 particles were tracked in each sample (n=6). 
• BPEI-50nm-10FBS-REP-1, BPEI-50nm-10FBS-REP-2, BPEI-50nm-10FBS-REP-3, BPEI-50nm-10FBS-REP-4/: particle tracks recorded for 50nm gold BPEI nanoparticles in DMEM supplemented with 10% FBS repeated 4 times (N=4). 6 particles were tracked in each sample (n=6). 
• Citrate-50nm-2.5FBS-REP-1*, Citrate -50nm-2.5FBS-REP-2*, Citrate -50nm-2.5FBS-REP-3*, Citrate -50nm-2.5FBS-REP-4*/: particle tracks recorded for 50nm gold Citrate nanoparticles in DMEM supplemented with 2.5% FBS repeated 4 times (N=4). 6 particles were tracked in each sample (n=6). 
• Citrate -50nm-5FBS-REP-1*, Citrate -50nm-5FBS-REP-2*, Citrate -50nm-5FBS-REP-3*, Citrate -50nm-5FBS-REP-4*/: particle tracks recorded for 50nm gold Citrate nanoparticles in DMEM supplemented with 5% FBS repeated 4 times (N=4). 6 particles were tracked in each sample (n=6). 
• Citrate -50nm-10FBS-REP-1*, Citrate -50nm-10FBS-REP-2*, Citrate -50nm-10FBS-REP-3*, Citrate -50nm-10FBS-REP-4*/: particle tracks recorded for 50nm gold Citrate nanoparticles in DMEM supplemented with 10% FBS repeated 4 times (N=4). 6 particles were tracked in each sample (n=6). 

10nm/:
• BPEI-10nm-2.5FBS-REP-1*, BPEI-10nm-2.5FBS-REP-2*, BPEI-10nm-2.5FBS-REP-3*, BPEI-10nm-2.5FBS-REP-4*/: particle tracks recorded for 10nm gold BPEI nanoparticles in DMEM supplemented with 2.5% FBS repeated 4 times (N=4). 6 particles were tracked in each sample (n=6). 
• BPEI-10nm-5FBS-REP-1, BPEI-10nm-5FBS-REP-2*, BPEI-10nm-5FBS-REP-3*, BPEI-10nm-5FBS-REP-4*/: particle tracks recorded for 10nm gold BPEI nanoparticles in DMEM supplemented with 5% FBS repeated 4 times (N=4). 6 particles were tracked in each sample (n=6). 
• BPEI-10nm-10FBS-REP-1, BPEI-10nm-10FBS-REP-2*, BPEI-10nm-10FBS-REP-3*, BPEI-10nm-10FBS-REP-4*/: particle tracks recorded for 10nm gold BPEI nanoparticles in DMEM supplemented with 10% FBS repeated 4 times (N=4). 6 particles were tracked in each sample (n=6). 
• Citrate-10nm-2.5FBS-REP-1, Citrate -10nm-2.5FBS-REP-2*, Citrate -10nm-2.5FBS-REP-3*, Citrate -10nm-2.5FBS-REP-4*/: particle tracks recorded for 10nm gold Citrate nanoparticles in DMEM supplemented with 2.5% FBS repeated 4 times (N=4). 6 particles were tracked in each sample (n=6). 
• Citrate -10nm-5FBS-REP-1, Citrate -10nm-5FBS-REP-2*, Citrate -10nm-5FBS-REP-3*, Citrate -10nm-5FBS-REP-4*/: particle tracks recorded for 10nm gold Citrate nanoparticles in DMEM supplemented with 5% FBS repeated 4 times (N=4). 6 particles were tracked in each sample (n=6). 
• Citrate -10nm-10FBS-REP-1, Citrate -10nm-10FBS-REP-2*, Citrate -10nm-10FBS-REP-3*, Citrate -10nm-10FBS-REP-4*/: particle tracks recorded for 10nm gold Citrate nanoparticles in DMEM supplemented with 10% FBS repeated 4 times (N=4). 6 particles were tracked in each sample (n=6).