This study examines the viability and functionality of hPCTS using different tissue culture formats. 
hPCTS were cultured for 15 days using an organotypic polytetrafluoroethylene Millicell insert, a CELLBLOKS® hydrophilic polyethylene terephthalate flow system plate or without an insert. 
Viability was assessed through MTS assays, while functionality was determined by measuring urea and albumin secretion across the 15 days in culture.