Data files associated with "Extremely fast amelioration of plasmid fitness costs by multiple functionally-diverse pathways" by Hall, Wright, Guymer, Harrison, and Brockhurst.

Hall, James (2019) Data files associated with "Extremely fast amelioration of plasmid fitness costs by multiple functionally-diverse pathways" by Hall, Wright, Guymer, Harrison, and Brockhurst. [Data Collection]

Data Catalogue DOI: 10.17638/datacat.liverpool.ac.uk/953

Original publication URL: https://doi.org/10.1099/mic.0.000862

Description

The acquisition of plasmids is often accompanied by high fitness costs such that compensatory evolution is required to allow plasmid survival. The mercury-resistance plasmid, pQBR55, drastically reduced the growth of its host, *Pseudomonas fluorescens* SBW25, immediately after acquisition, causing a small colony phenotype. We observed rapid restoration of the ancestral large colony morphology within just 48 hours of growth on agar plates, suggesting that compensatory mutations had occurred. Using genome sequencing we identified that restoration of the large colony phenotype was associated with chromosomal mutations in either an uncharacterised hypothetical protein PFLU4242, RNA polymerase, or the GacA/S two-component system. Targeted deletions in *PFLU4242*, *gacA*, or *gacS* recapitulated the ameliorated phenotype upon plasmid-acquisition. These data show that compensatory evolution can occur through three distinct mutational pathways and is fast enough to allow the survival of a plasmid despite it imposing very high fitness costs upon its host.

Keywords:

plasmids, horizontal gene transfer, compensatory evolution, amelioration, experimental evolution, Pseudomonas fluorescens

Divisions:

Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology

Depositing User:

James Hall

Date Deposited:

30 Aug 2019 09:46

Last Modified:

22 Oct 2019 13:43

DOI:

10.17638/datacat.liverpool.ac.uk/953